The determination of a small amount of a biological constituent by the use of chemiluminescence. X The determination of protein using a 1,10-phenanthroline-hydrogen peroxide-ruthenium(III) system.
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概要
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The catalytic activity of ruthenium(III) chloride in a chemiluminescence reaction with 1,10-phenanthroline–hydrogen peroxide has been found to decrease in the presence of protein. On the basis of this phenomenon, bovine serum albumin as a model protein was determined within the concentration range of 5×10<SUP>−5</SUP>–1×10<SUP>−1</SUP> g dm<SUP>−3</SUP> and a detection limit of about 1 ng.
- 公益社団法人 日本化学会の論文
著者
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Hara Tadashi
Department Of Chemical Engineering And Materials Sciences Faculty Of Engineering Doshisha University
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Tsukagoshi Kazuhiko
Department Of Chemical Engineering And Materials Science Doshisha University
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Tsukagoshi Kazuhiko
Department of Chemical Engineering, Faculty of Engineering, Doshisha University
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Imaki Masakatsu
Horiba Ltd.
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