The determination of a small amount of biological constituent by the use of chemiluminescence. IV. A highly sensitive detector for the HPLC of protein.
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概要
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A highly sensitive detector for the HPLC of protein has been manufactured by making use of the phenomenon that the catalytic activity of copper(II) against the chemiluminescence between luminol and hydrogen peroxide is lowered in the presence of protein. According to the present detector, 2×10<SUP>−4</SUP>–1×10<SUP>−1</SUP> g/dm<SUP>3</SUP> protein could be determined, with the detection limit of 10 ng. By combining the present detector with a TSK-GEL G3000SW column, 2×10<SUP>−3</SUP>–1×10<SUP>−1</SUP> g/dm<SUP>3</SUP> ovalbumin could be determined, with the detection limit of 50 ng. The ovalbumin in 2×10<SUP>−2</SUP> g/dm<SUP>3</SUP> could be determined by the internal-standard method with a coefficient of variation of 8.65% (<I>n</I>=5). Care had to be taken in the detection of the protein acting as a chemiluminescent catalyst.
- 公益社団法人 日本化学会の論文
著者
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Hara Tadashi
Department Of Chemical Engineering And Materials Sciences Faculty Of Engineering Doshisha University
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Ebuchi Takashi
Department of Chemical Engineering, Faculty of Engineering, Doshisha University
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Toriyama Motohiro
Department of Chemical Engineering, Faculty of Engineering, Doshisha University
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