Determination of a small amount of a biological constituent by the use of chemiluminescence. XII. Highly sensitive immunoaffinity chromatography.
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概要
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Highly sensitive immunoaffinity chromatography was established by combining a micro-bore affinity column with a chemiluminescence detector for protein monitored by a flow-injection method using a 1,10-phenanthroline–hydrogen peroxide–copper(II) system. After having optimized the bed volume of a column, the flow rate of a buffer solution which was used for an immune reaction, and the time necessary for an immune reaction in a column, human serum albumin in the range of 3×10<SUP>−4</SUP>–1×10<SUP>−1</SUP> g dm<SUP>−3</SUP> could be determined, with the detection limit of 15 ng as the injected amount. Human serum γ-globulin and bovine serum γ-globulin showed somewhat nonspecific adsorption onto an affinity column with values of 0.16 and 0.14 based on the specific adsorption 1.00 of human serum albumin onto a column, whereas bovine serum albumin, bovine serum α-globulin, and ovalbumin showed no nonspecific adsorption.
- 公益社団法人 日本化学会の論文
著者
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Hara Tadashi
Department Of Chemical Engineering And Materials Sciences Faculty Of Engineering Doshisha University
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Tsukagoshi Kazuhiko
Department Of Chemical Engineering And Materials Science Doshisha University
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Arai Akihiro
Department of Chemical Engineering, Faculty of Engineering, Doshisha University
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Iharada Takeshi
Department of Chemical Engineering, Faculty of Engineering, Doshisha University
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