Establishment of a Cell Line Persistently Infected with Chronic Wasting Disease Prions
スポンサーリンク
概要
- 論文の詳細を見る
Elk prion protein (PrPC) has been confirmed to be capable of rendering rabbit epithelial RK13 cells permissive to temporal infection by chronic wasting disease (CWD) prions. The present study satisfactorily generated persistently CWD prion-affected RK13 cells (RKC1-11) using elk PrPC expressing cells (elkRK13) that were generated via the lentiviral expression system with high efficiency. The elkRK13 cells have been shown to be permissive to accumulation of abnormal isoforms of prion protein (PrPSc) resulting from CWD prions up to 97 serial passages thus far. This novel prion-affected cell line will help facilitate investigation of the molecular basis of CWD prion pathogenesis and confirmation of CWD prion infectivity in vitro.
- 日本獣医学会の論文
著者
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横山 隆
(独)動物衛生研究所プリオン病研究センター
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YOKOYAMA Takashi
Prion Disease Research Center, National Institute of Animal Health
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横山 隆
農林水産省家畜衛生試験場
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Yokoyama Takashi
Department Of Chemistry Faculty Of Science Kyushu University
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Yokoyama Takashi
National Inst. Animal Health Ibaraki Jpn
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Yokoyama Takashi
Prion Disease Research Center National Institute Of Animal Health
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Yokoyama Takashi
National Institute Of Animal Health
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SOHN Hyun-Joo
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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KIM Min-Jeong
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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LEE Won-Yong
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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KIM Hyo-Jin
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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TARK Dong-Seob
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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LEE Yoon-Hee
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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CHO In-Soo
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency
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