Retrospective Analysis of Sheep Scrapie by Western Blotting with Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
スポンサーリンク
概要
- 論文の詳細を見る
An abnormal isoform of prion protein (PrPSc) was extracted from formalin-fixed paraffin-embedded (FFPE) tissues from sheep and analyzed by western blotting. PrPSc immunoreactivity against anti-PrP monoclonal antibody T2, which recognizes discontinuous PrP sequences, differed amongst individual scrapie sheep cases. This may reflect structural differences in PrPSc that have been formalin-fixed prior to their extraction. This study indicates that western blotting by using FFPE tissues is useful for the retrospective analysis of transmissible spongiform encephalopathies in which only formalin-fixed samples are available and in conducting transmissible spongiform encephalopathies surveillance where freezing system is insufficient.
- 日本獣医学会の論文
著者
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横山 隆
(独)動物衛生研究所プリオン病研究センター
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YOKOYAMA Takashi
Prion Disease Research Center, National Institute of Animal Health
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Mohri Shirou
Prion Disease Research Center National Institute Of Animal Health
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横山 隆
農林水産省家畜衛生試験場
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Yokoyama Takashi
Department Of Chemistry Faculty Of Science Kyushu University
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MASUJIN Kentaro
Prion Disease Research Center, National Institute of Animal Health
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OKADA Hiroyuki
Prion Disease Research Center, National Institute of Animal Health
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Yokoyama Takashi
National Inst. Animal Health Ibaraki Jpn
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Yokoyama Takashi
Prion Disease Research Center National Institute Of Animal Health
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Yokoyama Takashi
National Institute Of Animal Health
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DORJ Gantsetseg
Prion Disease Research Center, National Institute of Animal Health, Tsukuba 305-0856, Japan
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MIYAZAWA Kohtaro
Prion Disease Research Center, National Institute of Animal Health, Tsukuba 305-0856, Japan
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KIMURA Kumiko
Subtropical Disease Research Division, National Institute of Animal Health, Chuzan, Kagoshima 891-0105, Japan
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KIMURA Kumiko
Subtropical Disease Research Division, National Institute of Animal Health
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DORJ Gantsetseg
Prion Disease Research Center, National Institute of Animal Health
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MIYAZAWA Kohtaro
Prion Disease Research Center, National Institute of Animal Health
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MOHRI Shirou
Prion Disease Research Center, National Institute of Animal Health, Tsukuba 305-0856, Japan
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