Western blot assessment of prion inactivation by alkali treatment in the process of horticultural fertilizer production from meat meal(Fertilizers and Soil Amendments)

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Western blot detection of the abnormal isoform of the prion protein (PrP^<Sc> ) was used to assess prion inactivation by heating under alkaline conditions during the manufacturing process used to produce horticultural fertilizer from meat meal. PrP^<Sc> was detected in the sample prepared from the horticultural fertilizer spiked with a 0.25 μg equivalent of scrapie-infected mouse brain. In contrast, PrP^<Sc> was not detected in a sample containing 0.25 g brain equivalent prepared from a small-scale processed mixture of scrapie-infected mouse brain and meat meal using the same method as that used to produce horticultural fertilizer. Our results indicated that the amount of PrP^<Sc> decreased to at least 1/10^6 by processing with heating under alkaline conditions. Although the bioassay suggests that prion infectivity was reduced under these conditions, this procedure did not completely remove high-titer prion infectivity.
社団法人日本土壌肥料学会の論文