Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection
スポンサーリンク
概要
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Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. This research aimed to provide molecular evidence on the presence of this 2 species in Japan, and develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 Ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii, 13 I. ovatus) collected from 2 sites in Hokkaido, Japan were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The positive samples were then used to evaluate a newly developed gltA-based nested PCR method. Selected positive samples were further characterized using the groEL gene for confirmation and phylogenetic analyses. Two groups of sequence results were obtained: those which had closer identities with (1) A. phagocytophilum (99.5–99.6% for 16S rRNA, 97.5% for gltA, 98.4% for groEL), and with (2) Anaplasma sp. closely related to A. phagocytophilum in Japan (99.3% for 16S rRNA, 96.4–98.7% for gltA, 97.5–97.9% for groEL). The present study confirmed the distinct presence of A. phagocytophilum and its closely related Anaplasma sp. in Japan, and developed a new PCR detection method based on gltA that can distinguish the 2 organisms.
著者
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Inokuma Hisashi
Department Of Applied Veterinary Medicine Obihiro University Of Agriculture And Veterinary Medicine
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Yokoyama Naoaki
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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YBANEZ Adrian
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
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MATSUMOTO Kotaro
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
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KISHIMOTO Toshio
Okayama Prefectural Institute for Environmental Science and Public Health, Okayama 701–0298, Japan
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INOKUMA Hisashi
Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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MATSUMOTO Kotaro
Department of Applied Clinical Medicine, Obihiro University of Agriculture and Veterinary Medicine
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