First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
スポンサーリンク
概要
- 論文の詳細を見る
Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α, gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale from the Philippines revealed 99.1–100% (16S rRNA, gltA, groEL and Msp5) and 94.3–97.6% (Msp1α) identities to the closest isolates from other countries. Moreover, sequence analysis of the Msp1α gene showed 3 variants, including a case of co-infection with 2 variants. Phylogenetic analyses based on Msp1α and Msp5 genes revealed that Philippine A. marginale isolates formed a monophyletic lineage, and were phylogenetically related to Brazilian and Chinese isolates. On the other hand, a highly specific and sensitive nested PCR based on groEL, with a detection limit of 2 copies/PCR, was developed to detect A. marginale in the Philippines. This study reported the first molecular detection and characterization of A. marginale in cattle and R. microplus in Cebu, Philippines.
著者
-
Kawazu Shin-ichiro
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
Sivakumar Thillaiampalam
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
MATSUMOTO Kotaro
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
-
YBAÑEZ Adrian
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan
-
INOKUMA Hisashi
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan
-
YOKOYAMA Naoaki
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan
-
KAWAZU Shin-ichiro
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
-
YBANEZ Rochelle
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
-
RATILLA Jowarren
National Dairy Authority, Cebu City 6000, Philippines
-
PEREZ Zandro
GPY Veterinare Animale - Group of Veterinary Clinics, Cebu City 6000, Philippines
-
GABOTERO Shirleny
GPY Veterinare Animale - Group of Veterinary Clinics, Cebu City 6000, Philippines
-
HAKIMI Hassan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
-
MATSUMOTO Kotaro
Department of Applied Clinical Medicine, Obihiro University of Agriculture and Veterinary Medicine
関連論文
- Peroxiredoxins in malaria parasites : Parasitologic aspects
- Theileria sergenti感染牛における,末梢血単球の比率および実数の変化(短報)〔英文〕
- Membrane knobs of unfixed Babesia bovis-infected erythrocytes: new findings as revealed by atomic force microscopy and surface potential spectroscopy
- Establishment of an in vitro transgene expression system in epimastigotes of Trypanosoma congolense
- Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates
- Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
- Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan
- Comparison of Two Hemoplasma Species on Hematological Parameters in Cattle
- Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam
- Leishmania Mini-exon Genes for Molecular Epidemiology of Leishmaniasis in China and Ecuador
- Metastatic Breast Carcinoma Simulating Linitis Plastica of the Colon : Report of a Case
- Detection of the New Ehrlichia Species Closely Related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan
- Inhibitory effect of terpene nerolidol on the growth of Babesia parasites
- Target proteins of the cytosolic thioredoxin in Plasmodium falciparum
- A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African, and South American Countries
- Survey on Tick-Borne Pathogens in Thoroughbred Horses in the Hidaka District, Hokkaido, Japan
- Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection
- Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan
- First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
- A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and Candidatus Mycoplasma haemovis' in Hokkaido, Japan
- The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among Babesia bovis detected from Cattle Populations in Thailand, Brazil and Ghana
- PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam
- Effect of Chronic Hemoplasma Infection on Cattle Productivity
- Characterization of the Population Demographics and the MSP-1 Block 2 Allele Gene Frequencies of P. falciparum Infected Individuals in Davao, Philippines
- Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle
- A Polymorphism Observed in the Experimentally Successful Peptide Vaccine Sequence Derived from Theileria sergenti Piroplasm Major Surface Antigen (p33).
- Prevalence and Molecular Analyses of Hemotrophic Mycoplasma spp. (Hemoplasmas) Detected in Sika Deer (Cervus nippon yesoensis) in Japan
- Development of Monoclonal Antibodies That Target 1-Cys Peroxiredoxin and Differentiate Plasmodium falciparum from P. vivax and P. knowlesi