Testing for HPV-DNA in cervical intraepithelial neoplasm(CIN) - with the aim of the follow-up which was individualized-.

概要

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Several reports have indicated cervical cancer, commonly squamous cell carcinoma, arising from cervical intraepithelial neoplasm (CIN) and related to human papillomavirus (HPV) infection. About 30 types of HPV among 100 types which exist in the natural environment cause human genital organ infection. Several studies have established a strong relation between infection with the high-risk types of HPV and the development of cervical cancer and its precursors. In the current follow-up protocol, patients with cytological abnormalities are uniformly examined with periodical cytology in order to find progressive change. Introduction of testing for the high-risk types of HPV may keep to determine individual follow-up schedules. We investigated HPV-DNA by means of PCR using cytology specimens and in situ hybridization (ISH) of biopsy specimens of abnormal cytology patients. In 30 patients with abnormal cytology from class-III to class-IV, 29 patients were infected with HPV and 25 were high-risk types. Out of three patients in which ISH was performed, two patients showed positive staining in the nuclei of epithelial cells. Our results showed a high positive rate for infected HPV, which is similar to past reports. Testing of HPV-DNA for abnormal cytology cases can individualize the risk of patients and should be involved in follow-up protocol.