悪性腫瘍の免疫学的アプロ-チ-9-51Cr-release cytotoxicity assayを用いた頭頚部悪性腫瘍患者のnatural cell-mediated cytotoxicityについて

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Natural cell-mediated cytotoxicity of normal lymphocytes against cultured tumor cells in animals and humans has been reported. The overall concept of natural cytotoxicity was derived from the in vitro observation that lymphocytes of both healthy and patient donors exerted direct cytotoxic reactions against tumor-derived cells. In the present study, using a cultured cell line of maxillary cancer with the characteristics of epidermoid carcinoma as the target, it was demonstrated that lymphocytes of patients bearing malignant tumor in the head and neck region possessed cytotoxic activity. Mononuclear cells were separated by centrifugation on Ficoll-Hypaque gradients. Monocytes were removed by incubation at 37°C for one hour in a plastic dish with complete medium (RPMI 1640 and 20% FCS). After removal of monocytes, T cells and non-T cells were separated by the rosette forming technique using sheep erythrocytes. Whole lymphocytes from normal healthy donors exhibited higher cytotoxic activities with dose response reaction. On the other hand, patient lymphocytes showed weak activity, in paticular, at low E/T ratios.Removal of monocytes from lymphocyte suspension did not influence cytotoxicity. T cells showed extremely decreased cytotoxicity in comparison with non-T cells. It was suggested that the population of non-T cells mainly contributed in vitro to natural cytotoxic activity against malignant tumor cells.
耳鼻咽喉科臨床学会の論文