Studies on Transglycosidation to Vitamin B6 by Microorganisms : III. Distribution of Activity of Pyridoxine Glucoside Formation in Microorganisms

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The formation of pyridoxine glucoside was searched in various kinds of microorganisms which were preserved as type culture and isolated from natural materials. The result of screening showed that the activity was especially found in the bacteria belonging to genus, Sarcina and Micrococcus. The strains of Pseudomonas also produced pyridoxine glucoside-like compound from sucrose and pyridoxine in the culture filtrate.With the isolated and identified strain, Micrococcus sp. No. 431, several conditions on the formation of pyridoxine glucoside were investigated by growing culture. Sucrose and peptone were the excellent substrate as carbon and nitrogen sources. A few surfaceactive reagents showed the stimulative effect. The maxium values reached 1, 200μg per ml from 1, 000μg of pyridoxine per ml.
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