Effect of pulsing electromagnetic fields on growth-cartilage cells in culture.

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Recently, pulsing electromagnetic field (PEMF) stimulation has been used for the treatment of delayed unions and non-unions clinically, but the mechanism is not clearly known. As a result, we investigated the effect of PEMFs on costal growth-cartilage cells of rabbits in culture. Cell growth and the ability of DNA synthesis and glycosaminoglycan (GAG) synthesis in the cells were measured.A PEMF generator was made in this department by using Helmholtz coil. Repetitive pulse burst (RPB) with a burst width of 76ms, a pulse width of 230μs and 6.4Hz was passed through this coil. The magnetic field strength reached 4 Gauss at the point where culture dishes were positioned.Growth-cartilage cells were isolated from New Zealand White rabbits (_??_ 300-600g). Culture medium was HamF-12 with 10% FCS. The ability of DNA synthesis was measured by 3H-thymidine incorporation. The ability of GAG synthesis was measured by 35S-sulfuric acid incorporation.We concluded that PEMF stimulation tended to promote cell growth and metabolism, especially immediately after stimulation.
西日本整形・災害外科学会の論文