ウナギに寄生するPlistophora anguillarum胞子の極糸弾出条件〔ウナギのプリストホ-ラ病〕

概要

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In order to elucidate the conditions for extrusion of the polar filament of the microsporidian spore (Plistophora anguillarum), the effects of hydrogen peroxide, pH and temperature of incubation have been tested in vitro. The spores used for the study were collected from the diseased eels (Anguilla japonica) obtained at the Hamanako Branch of the Shizuoka Pref. Fish. Exptl. St. The results obtained are as follows: (1) In the case of H2O2, if a higher concentration was used, a higher rate of extrusion of the polar filament resulted. (2) The rate of extrusion showed high values at pH 3-4. This characteristic was never observed in other species. (3) On the other hand, related to temperature, the present species showed high rates of extrusion at low temperatures (5-10°C) unlike other species. (4) The high speed motion picture taken to observe extrusions showed the speed being about 7-8μ per 1/64 second. The length of the extruded polar filaments were 80-400μ (10-60 times that of the spores). (5) The polar filaments extruded were in a twisted form because it had remained coiled in the spore. (6) It was seen that the spores became dark grey after the extrusion.