サルアワビ筋肉中のプロテア-ゼ・インヒビタ-の性状〔英文〕

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A protease inhibitor, named tugalistatin, was isolated from the muscle of the marine gastropod Tugali gigas. Tugalistatin inhibited the proteolytic activity of trypsin and α-chymotrypsin. It also suppressed the activity of kallikrein as well as elastase to some extent. On the other hand, it was inactive against metallo-proteases and cysteine proteases. These suggest that tugalistatin is a serine protease inhibitor. In SDS-polyacryamide gel electrophoresis it showed a single band corresponding to 22, 000 under reduced conditions. The molecular weight of intact inhibitor was calculated to be 17, 000 when checked by FPLC on a Superose 12 column. Amino acid analysis revealed tugalistatin contained a large amount of glutamic acid (14.0%), half-cystine (11.1%), and aspartic acid (9.7%) in the molecule.
日本水産學會の論文