結核病巣内のmononuclear phagocytesの酵素活性,特にβ-galactosidaseおよびnaphthol AS-D acetate esteraseの変動

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Chronorogical changes in the activities of various enzymes of the mononuclear phagocytes (MN) in tuberculous lesions were studied histochemically to estimate the functions of MN at the stages of development and healing of the lesions in vivo. Dermal BCG lesions were produced in rabbit by the intradermal injection of BCG and the lesions were biopsied at various times after infection. The enzymes assayed were P-galactosidase, acid proteinase (cathepsin D like), hyaluronidase, Naphthol AS-D acetate esterase, acid phosphatase, succinic dehydroge nase and cytochrome oxidase. The MN stained * to +H+ by each enzyme were designated as positive cells.<BR>The activities of β-galactosidase, acid proteinase, hyaluronidase and esterase in MN varied by the stages of the lesions, i. e., the activities of the former three which were mostly found in the MN localized near to necrotic areas increased at the stage of development two to four week after infection and there after gradually diminished. The latter one, i.e., the activities of Naphthol AS-D acetate esterase were increased prominently in the MN of the healing lesions five to six weeks after infection.<BR>The cells with increased activities of the esterase gathered in multiple masses which are not concerned with necrotic areas or in all the areas of the granulation. The activities of acid phosphatase, succinic dehydrogenase or cytochrome oxidase were found evenly in the MN of any stage and were neither correlated with development nor healing of the lesions.<BR>Based on the studies of BCG lesions in rabbits at the stages of development and healing, human tuberculous lesions including a 7 days BCG lesion induced in a normal individual, a tuberculin reaction tested for patient with pulmonary tuberculosis and lymphnodes biopsied from patients with tuberculous lymphoadenitis were also studied by using the same histochemical methods. Only the cells with the activities of Naphthol AS-D acetate esterase showed similar behavior to the MN in experimental BCG lesions.<BR>In this paper, the significance of the Naphthol AS-D acetate esterase increase in MN at the stages of healing are discussed from the viewpoints of the healing mechanisms of tuberculous lesions.
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結核病巣内のmononuclear phagocytesの酵素活性,特にβ-galactosidaseおよびnaphthol AS-D acetate esteraseの変動

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