免疫された宿主における結核感染防御機構--初感染巣,再感染巣における活性化マクロファ-ジと14C標識BCGとの相互関係について

概要

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To evaluate immunopathologically the defense mechanism against tuberculosis infection in the immune host, the interrelation between activated macrophages (MN) and <SUP>14</SUP>C-labeled BCG was observed in BCG lesions. In normal and tuberculin-positive rabbits, multiple dermal BCG lesions were produced and biopsied at 3, 7, 10, 14 and 21 days after inoculation. A different lesion was used for each biopsy. The biopsies were quickly frozen and cut in a cryostat. The resulting tissue sections were stained for the lysosomal enzyme, β-galactosidase, which reflects the state of MN activation. Tubercle bacilli in the lesions were recognized both by staining with carbolfuchsin and by radioautography. The <SUP>14</SUP>C-label was of great help in detecting the bacilli and showing progressive digestion of them.
一般社団法人日本結核病学会の論文