Phagocytic Activity of Reticulo-endothelial Systems in Experimental Tuberculosis of Mice

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Concerning the non-specific resistance to tuberculosis, extensive studies were made by Nukadain Japan with his idea of "heterogenous resistance". Also recently Dubos and others reportedthat heterologous bacterial infection markedlyenhanced this resistance in mice.<BR>The establishment of the method of determiningthe function of reticulo-endothelial system (RES) by measuring the rate of clearance ofcarbon particles from the blood stream, hasmade it possible to study the relation betweenthe function of RES and the resistance to infectiousdiseases. In this connection, the authorsformerly reported a simplified method of measuringthe function of RES by use of Idia ink, which will be described as follows: Mice wereinjected intravenously with a does of 16mg ofcarbon particles per 100 gr of body weight.After 20 minutes o. 4 ml of blood was taken bycutting the axillary vessel, diluted with thesame amount of 3.8 % sodium citrate solution, and was subjected to the centrifugation. Thesupernatant fluid was further diluted with fivefoldvolume of distilled water. The amount ofcarbon contained in this sample was measuredelectrophotometrically. From this reading thelog of the true concentration of carbon particleswas calculated, of which the absolute value wasnamed "the phagocytic index of carbon particle"of RES.
一般社団法人日本結核病学会の論文