STC [2, 3-dipheny1-5-thienyl-(2)-tetrazolium chloride] の応用による結核菌発育の早期判定に関する研究 : 第4報STC含有培地の分離培養への応用に関する研究
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Authors reported the usefulness of STC for the earlier detection of mycobacterial growth. in previous papers (Kekkaku, Vol.46, p.335 and Vol.46, p.341). In these studies, 0.1m<I>l</I> of 0.5% STC solution (aqueous) was added onto the media after the inoculation. In the next paper authors reported the results of the application of STC-containing media in different concentration for earlier detection of mycobacterial growth and reported that 0.01% (100mcg/m<I>l</I>) was the appropriate concentration incorporating into the media (Kekkaku, Vol.47, p.269).<BR>This paper presents the results of isolation culture using 0.01% STC-containing media from sputa.<BR>The methods and results were summarized as follows:<BR>Experiment 1;<BR>Ogawas media modified by Kudoh were used for this study. STC was added into the media before inspissation to make 100mcg/ml (0.01%) STC-containing media. Sputa were treated with 4% NaOH (volume ratio 1: 4) and inoculated 0.1ml for a pair of original and STC containing media. The reading of bacillary growth was performed at 2, 3, 4, 6 and 8 weeks, after the inoculation.<BR>The results were shown in Table 1. As seen in this table, the difference in the culture positive rate between original and STC-containing media was not observed. Comparing the time of first growth between both media however 7.6% of positive culture on STC-containingmedia showed erlier growth than original media.<BR>Experiment 2;<BR>3% Ogawas media were used for this study. The pretreatment of sputa, the method of inoculation and the time of reading were the same as experiment 1.<BR>The results were shown in Table 2. The positive culture on original and STC-containing media was not different, however, the time of first growth was different on both media 14.6% ofpositive culture on STC-containing media showed earlier growth than on original media.<BR>The result of contamination was shown in Table 3. The contamination rate was not different significantly between original and STC-containing media.<BR>The niacin reaction using the stained colonies by STC was performed in small scale and reported in previous paper (Kekkaku, Vol.46, p.335). This time the niacin test using colonies isolated from each medium was performed and the results were shown in Table 4. As seen in this table, the difference of niacin reaction between colonies on original and on STC-containing media was not observed.<BR>In conclusion, 0.01% STC-containing media was useful for earlier detection of mycobacterial growthfrom pathological specimens.
- 日本結核病学会の論文
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