結核菌のThiacetazone耐性に関する研究 : 第3編ThiacetazoneとEthionamideの交叉耐性に関する研究

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The cross resistance between thiacetazone and ethionamide was reported by rr. any workers. This paper presents the ethionamide sensitivity of Japanese strains isolated from prevusly untreated tuberculous children according to different thiacetazone MIC. The results cf in vitro experiment using thiacetazone resistant H37Rv and ethi, : narnicie resistant H37Rv were also reported. The Dubos Tween Albumin culture of six strains which shcwed the different thiacetazone MIC- 0.25 mcg/ml MIC: No.3; 0.5 mcg/ml MIC: 8; 1 mcg/ml MIC: No.7; 2 mcg/ml MIC: No.10; 4 mcg/ml MIC: No.9; 8 mcg/ml MIC: No.6 were inoculated 10-3 mg and 10-5 mg o n ethionamide containing media -17?-; Ogawas mela. The drug cc, neentrations of etl.. ionarrirle tested were 12.5, 25 and 50 mcg/ml. The sensitivity fir thiacetazone and ethh, riarnicle of tha. cetazc, ne resistant H37Rv cr ethionamide resistant H37Rv was examined by using niedla. The baciary suspension was prepared by grinding the culture on 1% Ogayas media and by adding the sterile distilled water to get 1 mg/ml. The drug concentrations tested were as follows: thiacetaz.: 0. 1, 5, 10, 25, 50, 100, 250, 500 mcg/ml; ethionamide 0, 12.5, 25, 50, 100, 250, 500. 1, 000mcg/ml The inoculum size of test strains was 10-1, 10-3 and 105mg. The reading, of growth on the media was made at 3 and 4 weeks after the incculation.