STC [2, 3-diphenyl-5-thienyl-(2)-tetrazolium chloride] の応用による結核菌発育の早期判定に関する研究 : 第3報 STC含有培地に関する研究

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Authors reported the usefulness of STC for the earlier detection of mycobacterial growth in previous papers (Kekkaku, Vol.46, p.335 and Vol.46, p.341). In these studies 0.1ml of 0.5% STC solution (aqueous) was added onto the media after the inoculation. This paper presents the results of examination for the appropriate concentration of STC which was incorporated into the media before inspissation. The methods and results were summarized as follows: The concentration of STC which was incorporated into the media were 0.5, 0.1, 0.05, 0.01, 0.005, 0.002, 0.001 and 0.0002%; and 1% Ogawas media were used for examination. The strains used for study were H37Rv and Kurono; the inoculum size was 10-3 and 10-5mg. The reading of bacillary growth was performed at 7, 10, 14, 17, 21 and 28 days after the inoculation. The results were shown in Tables 1 and 2. As seen in these tables, the appropriate concentration of STC in media was 0.01% (100mcg/ml) for earlier detection of bacillary growth. On the media containing more than 0.05% of STC the bacilli showed dysgonic growth and on the media less than 0.002% of STC colonies showed only slight violet-red colouring. In conclusion, the appropriate concentration of STC in STC-containing media was 0.01% (100mcg/ml) and the application of STC-containing media for isolation culture should be amined.
日本結核病学会の論文