STUDIES ON THE TRANSITION OF SENSITIVITY FOR SM, INH AND PAS OF TUBERCLE BACILLI ISOLATED FROM THE PATIENTS TREATED BY TRIPLE COMBINATION CHEMOTHERAPY INCLUDING SM AND INH WITH SPECIAL REFERENCE TO THEIR CLINICAL COURSE

概要

論文の詳細を見る
Studies were made on the transition of sensitivity for SM, INH and PAS of tubercle bacilli isolated from the patients treated by triple combination chemotherapy including SM and INH. Thirty eight cases were selected from patients admitted to the author's sanatorium during the period from April 1, 1962 to November 30, 1963 according to the following criteria: 1) moderately or far advanced cases, 2) cases with definite cavity, 3) cases with positive bacilli both on smear and culture, 4) bacilli isolated from patients before the beginning of treatment showed no or few growth (less than 1% with that on the control media) on the media containing 10 Jug Iml SM and on the media containing 0.1μg/ml INH, 5) cases treated by triple combination chemotherapy including SM and INH after admission. Sensitivity of tubercle bacilli isolated from these cases before and during treatment was examined by the following procedures: 1) Media: Ogawa media, 2) Concentration of drugs a) SM: each medium contains 2, 10 and 20 μg before coagulation, and because of absorption, actual concentration in each medium is estimated at 1, 5 and 10μg/ml, b) INH: 0.025, 0.05, 0.1, 0.5 and 1μg/ml, c) PAS: 0.1, 0.5 and 1 μg/ml, 3) Inoculation of bacilli: Bacillary suspension of 1 mg/ml was prepared from the isolation culture or subculture by using grainding flask. Suspension was diluted, and 10-3, 10-4 and 10-5 mg of tubercle bacilli were inoculated on the series of each 2 test media. The transition of sensitivity for drugs was studied with special reference to the transition of bacillary findings and the changes in roentgenological findings, and the results were summarized as follows: 1. Among 38 cases, 34 cases converted to negative both on smear and culture during chemotherapy. 2. No marked changes in the sensitivity of tubercle bacilli for the 3 drugs was demonstrated during chemotherapy among cases converted to negative. As shown in Table 1, the rate of strains showing more than 10% growth on the media containing 1 μg SM and that showing more than 50% growth on the media containg 0.025 μg INH and 0.1 μg PAS, were nearly equal during chemotherapy. 3. Among cases converted to negative, no growth of bacilli was observed in 85 of 89 strains on the media containing 5 μg SM, in 88 of 90 strains on the media containing 0.1 μg INH, and in 56 of 62 strains on the media containing 1 μg PAS (Table 2). The growth rate of bacilli grwon on the media containing 5 μg SM compared with that on the control media was less than 3%, and that on 0.1 μg INH was less than 30%, and that on 1 μg PAS was less than 1%.
一般社団法人日本結核病学会の論文

STUDIES ON THE TRANSITION OF SENSITIVITY FOR SM, INH AND PAS OF TUBERCLE BACILLI ISOLATED FROM THE PATIENTS TREATED BY TRIPLE COMBINATION CHEMOTHERAPY INCLUDING SM AND INH WITH SPECIAL REFERENCE TO THEIR CLINICAL COURSE

スポンサーリンク

概要

著者

関連論文

スポンサーリンク