asマーカーによるヒト型結核菌H37Rv株とH37Ra株とのin vitro分別

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Some H37 Rv and H37 Ra strains of human-type tubercle bacilli were supplied by the Research Institute for Microbial Diseases, Osaka University; the Research Institute for Tuberculosis and Leprosy, Tohoku University; and the National Institute of Health, Japan. They were inoculated to Ogawa acid egg-solid medium at pH 4.5 in order to differentiate an avirulent strain from a virulent one of humantype tubercle bacilli in vitro. Tentatively, strains which grew well on the acid egg medium were referred to as as+ (no acid-sensitive) ones, and those which could not grow on the same medium as as- strains.1. When three H37 Rv strains were made into crude bacillary suspensions and inoculated, all of them grew well on the acid egg medium, whereas three H37 Ra strains did not grow on this medium. In other words, the former were as+ and the latter as-. Therefore, it is evident that avirulent strains of human-type tubercle bacilli may easily and certainly be distinguished from virulant ones in vitro by the aid of as marker.2. It was found, however, that the H37 Rv strain contained an as- population at the rate of 10% and the H37 Ra strain an as+ population at the rate of 40%.3. From among the H37 Rv and H37 Ra strains, one colony each having typical properties characteristic of the respective strain was selected. This colony was cloned further into 50 colonies. Fifty colonies of each strain were examined by the aid of as marker. Consequently, it was demonstrated that colonies having as- marker were present in the typical H37 Rv colony selected at the rate of 6%, and that the colonies obtained from the H37 Ra strain which had been regarded as a typical one contained an as+ population at the rate of 8%.4. On the other hand, colonies selected from among the H37 Rv and H37 Ra strains and having a typical characteristics contained an as+ population at the rate of 92% and no as- population, respectively.From the results mentioned above, it is presumed that the fundamental properties of the H37 Rv and H37 Ra strains may commonly be as+, although the phenotypes of the H37 Rv and H37 Ra strains are as+ and as-, respectively.
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asマーカーによるヒト型結核菌H37Rv株とH37Ra株とのin vitro分別

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