鼠癩菌の生物学的性状に関する研究-2-

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The Kumamoto strain of Myc. lepraemurium was suspended in M/20 Sörensen buffer (pH 7.0) con-taining a different kind of animal serum, that is, mouse, guinea pig, horse, cattle, rabbit, goat, sheep, and monkey, respectively, and was incubated at 37°C. for 30 days. Infectious activity of Myc. leprae-murium in each medium was tested by murine leprosy production; namely, two tenth ml of each medium containing the bacterial suspension was inoculated subcutaneously to mice after 10, 20, and 30 days of in-cubation, respectively, and mice tested were killed approximately five months after inoculation.<BR>From the results obtained here, it would be presumed that cattle serum would be best, among sera tested, for maintaining the infectious activity of Myc. lepraemurium, because the bacterial suspension stored even for 30 days at 37°C. in the medium containing cattle serum could produce murine leprosy, whereas the infectivities of the bacilli stored in the medium containing other sera were lost for 20 days' incubation at 37°C.
日本細菌学会の論文