ビタミンB12の乳酸菌への吸着条件に関する研究

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It might be presumed that one of the possible etiologies of diarrhoe caused by non-pathogenic bacterial substance would be that some kind of enterobacteria of bacterial flora in intestinal tract absorbs VB12 which is necessary for digestion, prior to combination with intrinsic factor. Therefore, absorption conditions of VB12 to the bacterial cells were studied in order to clarify a possible etiology of diarrhoe.Several factors affecting absorption of vitamin B12 by Lactobacillus leichmannii ATCC 4797 which requires VB12 for growth were determined by radioactive measurement, comparing to VB12 non-required other lactobacilli. Whole cells and cell wall fraction were used throughout the studies. The results obtained were as follows.1. VB12 were absorbed specifically to the whole cell and cell wall of L. leichmannii for very short time and at low temperature, however, there was no complete relationship of absorption rate between dosage of vitamin B12 and numbers of bacterial cells.2. Properties of VB12 absorption by cell wall were somewhat different from that by whole cells. These differences might be due to different characteristic of inner side of cell wall from out side of the cell.3. Absorption of VB12 by the bacteria were inhibited by heating at 100°C, by treatment with formalin, ethanol and chloroform, and by digestion with trypsin, lipase as well as amylase, but were markedly stimulated by digestion with papain.4. VB12 absorption to the cell wall was significantly stimulated in the presence of salts, whereas there were no effects of salts in the cases of whole cells.5. No specific absorption of VB12 by VB12 non-required strain of lactobacilli occurred, even if the bacilli were administered by various treatments which might possibly convert from VB12 insusceptible cells to susceptible ones.
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ビタミンB12の乳酸菌への吸着条件に関する研究

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