Determination of .ALPHA.-Tocopherol Stereoisomers in Biological Specimens Using Chiral Phase High-Performance Liquid Chromatography.

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The α-tocopherol stereoisomers in biological specimens were investigated using high-performance liquid chromatography. All-rac-α-T oc acetate was separated into four peaks (peak area ratio: 4:2:1:1) by Chiralpak OP(-f-) HPLC. 2R-isomers constituted the first peak and 2S isomers were separated into three peaks (peak area ratio:2:1:1). 2-Ambo-α-T oc acetate was completely separated into RRR- and SRR-a-Toc acetate by this method. The present HPLC method was used for the separation of all-rac-α-Toc in blood and tissues of rat. The analytical recoveries of RRR- and SRR-a-Toc acetate added to blood and tissues were 90.5-98.2% for RRR-a-Toc and 93.7-100.5% for SRR-α-Toc. The distribution of α-Toc stereoisomers in the blood and tissues from rats administered all-rac-a-Toc acetate was investigated by HPLC. The concentrations of the 2R-isomers in the blood and tissues were markedly higher than the concentrations of the 2S isomers, and the levels of a-Toc stereoisomers showed marked differences between blood and tissues.
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