Establishment of normal epithelial cell lines from mouse submandibular gland in serum-free cell culture.

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Normal epithelial cell line (M-SGα) has been established from mouse submandibular gland in serum-free cell culture. Although the mode of chromosome numbers of the cells shifted from diploid to triploid with increasing passage level, the cells had no tumorigenicity in athymic nude mice. M-SGα expressed some of the functional proteins which were characteristic of mouse submandibular gland ductal cells by indirect immunofluorescence antibody technique and also expressed EGF, FGF and TGFβreceptors. Proliferation of the cells was stimulated by either EGF or acidic-fibroblast growth factor (a-FGF). Furthermore, it was revealed that EGF and FGF were essential factors for the growth. Growth of the cells was inhibited by calf serum or transforming growth factor β(TGFβ). But the inhibitory activities in calf serum were destroyed by heat treatment. Therefore, it was speculated that some unknown growth inhibitor for epithelial cells which was different from TGFβexisted in calf serum.<BR>Taken together, this cell line which was established in serum-free culture would be useful to study the growth and differentiation of normal epithelial cells.
社団法人日本口腔外科学会の論文

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