Establishment of normal diploid epithelial cell lines from mouse submandibular gland in serum-free cell culture.
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Normal epithelial cell lines from mouse submandibular glands have been established in serum-free medium with EGF (M-SGβ1 cell line) or acidic-FGF (a-FGF)(MSGβ2 cell line).<BR>These cell lines were non-tumorigenic in athymic nude mice and showed some of the functional proteins, characteristic to the mouse submandibular gland, such as secretory component, amylase and mouse EGF. Furthermore, it has been revealed that EGF and a-FGF stimulate proliferation of M-SGβ1 and M-SGβ2 dose dependently and have the same function on the cells affecting growth.<BR>On the other hand, it has been found that the chromosome number of M-SGβ2 remained diploid, but that of M-SGβ1 shifted to triploid. Furthermore, culturing with EGF, chromosome number of the cells rapidly shifted from diploid to triploid. These results suggested that a-FGF but not EGF, has an important funciton of keeping the chromosome number diploid.<BR>M-SGβ2 which is a diploid epithelial cell line would be very useful for the study of growth factor expression and their receptors in the process of normal epithelial cell malignant transformation.
- 社団法人 日本口腔外科学会の論文
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- Establishment of normal diploid epithelial cell lines from mouse submandibular gland in serum-free cell culture.
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