Herpes simplex virus infection of human oral mucosa in cell culture and organ culture systems.

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Herpes simplex virus (HSV)-human oral mucosa interaction was investigated using cultured cells and organ cultures. Epithelial cells and fibroblasts derived from human oral mucosa and mucosa tissues were infected with HSV in vitro and the expression of HSV antigen was examined by immunofluorescent antibody technique. Cultured epithelial cells and fibroblasts showed degenerative change in 24 hours after infection and HSV antigen was demonstrated in almost all of the cells. On the other hand, in organ cultures, examined 3 days post infection, the appearance of HSV antigen was confined to the undifferentiated epithelial cells of the basal cell layer and lower prickle cell layer. Neither differentiated epithelial cells nor fibroblasts in the subepithelial connective tissue showed HSV antigen. When the epithelial cells freshly dissociated from epithelium by trypsin treatment were infected with HSV and were cultured for 3 days, viral antigen was detected in 3.0-17.1% of the cells. Double-irru-nunostaining of these epithelial cells revealed that laminin-positive cells showed HSV antigen, whereas HSV antigen was not found in the cells containing highmolecular-weight keratin. These findings indicate that differentiation state of the epithelial cells plays an important role in the appearance of HSV antigen and that once grown in the in vitro culture system, fibroblasts in the subepithelial connective tissue will allow HSV replication.
社団法人日本口腔外科学会の論文

Herpes simplex virus infection of human oral mucosa in cell culture and organ culture systems.

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