In vitro isolation and immunocytochemical characterization of tumor cells derived from a recurrent lower gingival carcinoma with sarcomatous changes.

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We previously reported a rare case of recurrent gingival carcinoma with sarcomatous changes occurring after chemotherapy and irradiation as initial treatment. Immunohistochemical staining by the avidin-biotin-peroxidase complex (ABC) method for keratin and vimentin revealed that the primary tumor diagnosed as squamous cell carcinoma was only positive for keratin while the recurrent tumor was positive for both keratin and vimentin.<BR>In this paper, we report the <I>in vitro</I> isolation of a tumor cell line, designated as SCCHT from the recurrent tumor. SCCHT was short, spindle shaped with dendrite-like processes in culture. This cell line had colony forming ability in semisolid agar and produced a tumor by inoculation into nude mice, which was diagnosed as squamous cell carcinoma with sarcomatous changes, similar to the surgical specimen. I mmunocytochemical staining detected keratin and vimentin but not desmin in SCCHT and its tumor in nude mice. These findings were confirmed by Western blot analysis performed using two oral squamous cell carcinoma cell lines, SCCKN and SCCTF, and gingival fibroblasts as controls. SCCHT demonstrated a broad band of keratin ranging from 56 kDa to 64 kDa similar to SCCKN and SCCTF, but showed four bands of vimentin at 57, 52, 47 and 43 kDa in contrast to the 57 kDa band obtained for control fibroblasts.<BR>These results indicated that SCCHT is a squamous cell carcinoma cell line which possesses keratin and vimentin, resulting in sarcomatous changes in morphology.
社団法人日本口腔外科学会の論文

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