ウレアーゼを固定化した陽イオン交換カラムを用いる尿中尿素のイオンクロマトグラフィー

概要

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A quantitative method for the determination of urea in human urine was studied. An ion chromatograph (IC) with a conductivity detector was used in this method, where the chromatograph was modified by placing a cation exchanger-an immobilized urease column on the injection loop with a 6-way valve in IC. A cation exchanger was prepared by the treatment of cedar sawdust with fuming sulfuric acid (exchange capacity, 1.2 meq g-resin−1). Immobilized urease was prepared by the adsorption of urease on cedar sawdust with triethylenetetramine (activity, 3500 U g−1). The reactor with a cation exchanger-an immobilized urease column could convert urea in urine to ammonium ion (conversion efficiency, 100%) even if a sample solution was passing through column at a 9 mL min−1 rate. Simultaneously, the above-mentioned reactor could also completely hold NH4+, Na+ and K+ in urine. The calibration curves of urea were very linear over 4.0 – 20 mg L−1. The urea concentration in urine could be determined within 7 min after developing the urine sample.