いわゆる低分子量グルテニンのポリペプチド構成について

概要

論文の詳細を見る
The polypeptide composition of low molecular weight glutenin (LMWGn) described by Beckwith et al.(4) was compared with that of the usual glutenin (Gn). Starch gel electrophoresis of reduced and cyanoethylated glutenin (SCE-Gn) and low molecular weight glutenin (SCE-LMWGn) showed that SCE-LMWGn lacked a few components of SCE-Gn. While SCE-Gn was separated into three fractions by gel filtration on Sephadex G-100 column, SCE-LMWGn lacked the second fraction in the same fractionation. The second fraction of SCE-Gn gave three main bands in starch gel electrophoresis. The same components were found in the preciptate when the fractionation of SCE-Gn was made in neutral 70% ethanol. On the other hand, starch gel electrophoretic pattern of the supernatant was similar to that of SCE-LMWGn, which gave no precipitate in neutral 70% ethanol at room temperature. Isoelectric focusing revealed another difference between SCE-Gn and SCE-LMWGn; the latter lacked all of the components of SCE-Gn having isoelectric points lower than 6.4. These lacked components were found in the neutral 70% ethanol insoluble fraction of SCE-Gn and in the first fraction of gel filtration. The components of the second fraction in gel filtration showed isoelectric points higher than 6.4.
社団法人日本農芸化学会の論文