肥後ギクのin vitro保存〔英文〕

概要

論文の詳細を見る
In order to establish a method for the preservation of Higo chrysanthemum cultivars (Dendranthema × grandiflorum (Ramat.) Kitam.), studies were conducted on the influences of the type of explant, temperature, light and other conditions on the survival and growth of cultures in vitro. Cultures at 5 °C, 15 °C, 25 °C and 25°-15°-5 °C had maximum rates of 100%, 60%, 53% and 100% of survival, respectively, for a period of 12 months. Cultures at 15 °C and 25 °C showed a comparatively active growth and advanced senescence or withering after 12 months of incubation. On the other hand, cultures at 5 °C and 25°-15°-5°C showed growth retardation and delayed senescence which had maximum rates of 100% and 75% survival, respectively after 24 months. Dark condition increased the survival rate of cultures at 5 °C although the plant height was comparatively smaller than that under light. Nodal segments showed higher survival rate than the apical buds, and the removal of the expanded leaf on the explant showed advantages upon incubation. One ml of MS medium without growth regulators in a 12 × 75 mm test tube was enough to ensure the survival of a culture at 5 °C for a period of 24 months without transferring to a new medium or without supplementing the medium. Based on the above results, a practically safe, economical and useful system for the in vitro preservation of Higo chrysanthemum has been established. This is presently applied to the 21 cultivars.
園藝學會の論文

肥後ギクのin vitro保存〔英文〕

スポンサーリンク

概要

著者

関連論文

スポンサーリンク