糸状菌による脂質の生産に関する研究 (第12報) : Fusarium moniliforme のアルカンあるいはグルコースを炭素源とした培養条件による脂質組成の変動

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The influence of growth temperature, C/N ratio of the medium and nitrogen source on the amounts of lipids formed from alkane or glucose in the mycelium of Fusarium moniliforme S-13 strain was invesigtated. Lipid content in the mycelium of the strain increased with increasing C/N ratio, particularly when using glucose as the carbon source. The greatest amount of lipids (53.4%) was obtained at a C/N ratio of 343 with ammonium nitrate and glucose as the nitrogen and carbon source at 30°C. The maximum accumulation of lipids using decane and glucose occurred at a C/N ratio of 24.1 at 20°C with ammonium sulfate (0.43g/400ml of meidum) and at a C/N ratio of 114 at 30°C with ammonium nitrate (0.96g/400ml of medium) respectively. The fatty acid composition of neutral and polar lipid fractions fractionated from the lipid extracts by silicic acid column chromatography and the lipid composition of both lipid fractions were determined. Changes in composition by variation in culture conditions, such as the use of different carbon sources, were also investigated.
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