糸状菌による脂質の生産に関する研究(第8報) : Mortierella isabellina(2菌株)の脂質組成に及ぼす培養条件の影響

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The influence of cultural conditions, especially C/N ratio of medium, growth temperature and nitrogen source, on the amounts of lipids formed from, glucose in the mycelium of two strains(IFO 7884 and 7824) of Mortierella isabellina was investigated. It was found that the contents of lipids in the mycelium of the strains increased with increasing C/N ratio. The highest content(83.5%) of lipids was obtained at a C/N ratio of 343 at 20°C by the strain when NH4NO3 was used as a nitrogen source. The maximum accumulation of lipids, 1.68g/400ml medium, occurred at a C/N ratio of 11.4at 20°C by using (NH4)2SO4. Ammonium nitrate was the best source of nitrogen for production of lipid by these strains. In this case, the totol intracellular lipids produced was 14.0g/100g of glucose. The fatty acid compositions of neutral and polar lipid fractions fractionated from lipid extracts by silicic acid column chromatography and the lipid compositions of both fractions were determined. The influence of the cultural conditions on these compositions was also investigated.
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