糸状菌による脂質の生産に関する研究 (第7報) : 高速液体クロマトグラフィーによる微生物極性脂質の定量

概要

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A rapid and efficient method for the separation of polar lipids in microorganisms by high-performance liquid chromatography was developed. The chromatography of polar lipids was perfomed on a system combined with two columns (Zorbax CN and ODS) using acetonitrile-water-phosphoric acid (100 : 2 : 0.1, by vol.) as a moving solvent and an UV detector (absorbance at 203nm). A complete sepaLration and quantitation of polar lipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, glycolipid containing phosphorus and glycolipid were achieved by this method using ergosterol as an internal standard. These lipids were main components of polar lipids in the microorganisms such as <I>Mortierella isabellina, Pellicularia filamentosa</I> and <I>Fusarium moniliforme</I>.
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