糸状菌による脂質の生産に関する研究(第9報) : Mortierella isabellina(2菌株)のステロール組成及びスクアレン含量に及ぼす培養条件の影響

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The influence of cultural conditions, especially C/N ratio of medium, growth temperature and nitrogen source, on the amounts of sterols and squalene farmed from glucose in the mycelium of two strains of <I>Mortierella isabellina</I>(IFO 7884, IFO 7824) was investigated. The contents of sterols in the mycelium of the strains varied between 0.20.8% of the dry cell weight by cultural conditions. The greatest amount of sterols, 43.1mg/400ml medium, was observed in M.isabellina IFO 7884 grown at a C/N ratio of 11.4at 30°C by using urea as a nitrogen source. The greatest amount of squalene, 5.9mg/400 ml medium, was obtained at a C/N ratio of 11.4 at 3O°Cin <I>M. isabellina</I> IFO 7884 by using ammonium sulfate as a nitrogen source. The influence of the cultural conditions on the qualitative and quantitative compositions of sterols, was investigated. Ergosterol, ergosta-5, 7-dienol, and 24-methylenedihydrolanosterol were major sterols in the strains.
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