メチル水銀投与ラットにおける脂質過酸化および脂肪酸組成

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Four groups of rats, with 5 animals in each, were fed a diet to which 1, 2, 4 or 8ppm of methylmercury (MeHg) were added while another group of 5 rats was fed a diet containing no MeHg as a control. Moreover, each rat was daily supplied the same volume of these diets. During the experimental period of 40 days, body weights of the rats were daily checked individually. On day 40, expired air of the rats was collected to analyse for ethane and pentane production, and then the rats were sacrificed to obtain the liver, kidneys, brain and plasma. Thio-barbituric acid (TBA)-reactants in the selective organs and plasma were measured to investigate the effects of MeHg on lipid peroxidation. Further, gaschromatographic analyses of fatty acids were carried out on the samples of the selective organs and plasma to examine the effects of MeHg on lipid metabolism. Results obtained were as follows:1. The fifth day after the experiment started, the growth rates of the groups fed a diet containing MeHg (the MeHg groups) were higher than those of the control group. Moreover, the growth rates tended to increase with increases of MeHg concentrations in food from days 25 to 32 of the experimental period. Furthermore, the weights of the kidneys increased with increases in MeHg concentrations in food.2. The amounts of expired ethane and pentane did not increase proportionally with increases in MeHg concentrations in food but those of expired ethane and pentane were higher in the MeHg groups than in the control group.3. Higher concentrations of TBA-reactants in the liver, kidneys and brain, and plasma, except for the liver of the group fed a diet with 1ppm of MeHg added, were observed in the MeHg groups when compared to the control group. The concentrations of TBA-reactants in the kidneys increased with increases in MeHg concentrations in food.4. The concentrations of polyenic acid of the ω-3 series (ω-3) in plasma and those of the ω-6 series (ω-6) in the liver increased, while those of monoenic acids in the kidneys decreased with increases in MeHg concentrations in food. In the organs and plasma, except for in the brain, the correlation coefficients between the concentration of polyenic acid and the concentration of the reference TBA-reactants were not significant.5. The ratios of linolate/arachidate both in plasma and in the liver, monoenic acids/saturated acids in the kidneys, and arachidate/docosatetraenoate in the brain were decreased with increases in MeHg concentrations in food, while those of ω-3/ω-6 in plasma were increased with that factor.6. The possible induction of lipid peroxidation and the changes of fatty acid composition in the organs and plasma by methylmercury was discussed in relation to the mechanism by which the development of neurological disturbance in MMC poisoning might occur.

メチル水銀投与ラットにおける脂質過酸化および脂肪酸組成

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