Radioimmunoassay for 11-Deoxycortisol Using Iodine-Labeled Tracer
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概要
- 論文の詳細を見る
A simple and sensitive radioimmunoassay for 11-deoxycortisol was developped. The antiserum produced in rabbits by immunizing with a complex of 11-deoxycortisol-3-oxime and bovine serum albumin (BSA) has little cross-reactivity with other endogenous steroids. The immunoassay procedure requires only one-step ethanol denaturation of binding proteins in plasma and extraction by an organic solvent can be omitted. Furthermore, use of <SUP>125</SUP>I-labeled tracer significantly simplify the counting procedure. The method is sensitive enough to detect 1μg/100m<I>l</I> of 11-deoxycortisol.<BR>Plasma 11-deoxycortisol levels measured by this method after the administration of a single dose of metyrapone ranged from 5.0 to 19.2μg/100m<I>l</I>, whereas they were 0 to 4.0μg/100m<I>l</I> in hypopituitary patients.<BR>It is concluded that this simple method is useful for the routine assay of plasma 11-deoxycortisol as a parameter of the metyrapone tests.
- 社団法人 日本内分泌学会の論文
著者
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Matsukura Shigeru
Third Department Of Internal Medicine Miyazaki Medical College
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SAKAMOTO NOBORU
Third Division, Department of Medicine, Kobe University School of Medicine
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Imura Hiroo
Third Division Department Of Medicine Kobe University School Of Medicine
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TSUBOI SEIKICHI
Third Division, Department of Medicine, Kobe University School of Medicine
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TOKUMIYA TOSHINOBU
Third Division, Department of Medicine, Kobe University School of Medicine
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TACHIBANA SEIJI
Daiichi Radioisotope Labomtories
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