High Molecular Weight Forms of Immunoreactive ACTH in a Human Pituitary and Ectopic ACTH-Producing Tumors

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High molecular weight forms of immunoreactive ACTH (IR-ACTH) were studied in a human pituitary gland and 4 ectopic ACTH-producing tumors in man. Both the pituitary and tumor extracts contained “big” IR-ACTH, which eluted near the void volume, and a small amount of “intermediate” IR-ACTH components, which eluted between the void volume and 125I-αh1-39ACTH, in addition to “little” ACTH which coeluted with 125I-αh1-39ACTH by Sephadex G-100 gel filtration. A significant amount of the “big” IR-ACTH applied bound to the concanavalin A-agarose column and was eluted with 0.2M α-methyl-D-mannopyranoside, indicating the glycoprotein content of “big” IR-ACTH fractions. When the “big” and “intermediate” fractions were further analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were resolved into 4 molecular forms of IR-ACTH with apparent molecular weights of 37, 000, 24, 000, 18, 000 and 4, 500, respectively. These results indicate that 3 high molecular forms of IR-ACTH are present in the human pituitary and the ectopic ACTH-producing tumors.
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