生体外培養によるウナギ卵の成熟促進と卵の初期発生

概要

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Ovarian eggs of the Japanese eel, Anguilla japonica, which were brought to the migratory nucleus stage by administration of a suspension of dog salmon pituitary glands, could be induced to mature by in vitro culture with synthetic medium 199 added to calf serum or a similar medium containing gonadotropic hormones. Ripe eggs were spherical in shape, measured about 1.0 to 1.26mm in diameter, and contained a large oil drop in the ooplasm of the vegetative pole. These eggs were pelagic in nature. About 30 minutes after insemination, fertilized eggs formed a moderate perivitelline space and egg cleavage began about two hours later. Cleavage was of the typical discoidal type. Eggs continued to develop until the 42nd hour and formed embryos of the morula, blastula and gastrula stage. These embryos displayed morphological features commonly observed in teleost fishes.
公益社団法人日本水産学会の論文