腸炎ビブリオおよびその類縁菌における菌体内溶血因子のリボヌクレアーゼ,トリプシン処理による活性化

概要

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The effect of ribonuclease and trypsin treatment on the intracellular haemolytic factor was investigated employing strains of Vibrio parahaemolyticus and related organisms. Nine fractions were derived from dried cells of each of the strains subjected to water extrac-tion plus enzyme treatment. The resultant insoluble fraction of a strain of V. parahaemoly-ticus was separated into three subfractions (A, B and C) of cellular debris by density gradient centrifugation. The haemolytic activities of these fractions were estimated quantitatively and the results are summarized as follows. (1) Haemolytic activity was not found in any of the soluble fractions. (2) The activity was found in dried cells and also in the water insoluble frac-tions of the Kanagawa phenomenon-positive strains, but not in those prepared from a negative strain. There were activity increments in both the Kanagawa phenomenon-positive strains and the single negative strain which were subjected to trypsin digestion. (4) Among the three fractions, A, B and C, A and B showed higher activities than C.
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