海水のビタミンB12に関する研究-II : 海水のビタミンB12定量法並びに海洋におけるB12分布

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In the preceding paper, the authors reported on a determination method of vitamin B12 in sea water, in which EDTA and an ion-exchange resin (Amberlite IR 4 B) were adopted to remove substances disturbing growth of test organism, such as Cl and Mg, from the test sea water. This procedure, however, being somewhat troublesome, a more simple one was investigated. After several trials it was ascertained that the disturbing substances are effectively removed be dialysis with cellophane within the lapse of 15 hours without any recognizable loss of the vitamin (Fig. 1 and Tab. 1) and that the test solutions ready for microbiological assay using Euglena are easily obtainable with this procedure. Using this method, vitamin B12 in sea water collected by the “Kagoshima-maru” at stations in the North Pacific from Lat. 0° to 30° N on the line of Long. 130°E, was analyzed and the result is shown in Fig. 2.
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