Interaction of a Fusogenic Liposome with HeLa Cell.
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概要
- 論文の詳細を見る
A plasmid, pActDTA, whose expression is fatal to mammalian cells, caused a decrease in HeLa cell survival only if the plasmid was encapsulated in egg PC liposome whose surface was modified with a poly(ethylene glycol) derivative bearing two alkyl chains (abbreviated as PEG-lipid). This result suggests that the reconstitution of PEG-lipid into the liposome made direct transport of the plasmid into the cytosol of the cell by a mechanism other than endocytosis possible. Furthermore, a transmission electron micrographic observation showed that the interaction between HeLa cell and the PEG-lipid reconstituted liposome was consistent with fusion. This interaction was unique to the PEG-lipid reconstituted liposome. These results strongly suggest that this be the first example of PEG-lipid induced fusion of liposome to a mammalian cell and support the validity of the PEG-lipid reconstituted liposome as a fusogenic liposome to a mammalian cell.
- 日本学士院の論文
著者
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Okumura Yukihisa
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
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Yamauchi Masahiro
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Yamamoto Minoru
Department Of Ecosystem Engineering The University Of Tokushima
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SUNAMOTO JUNZO
Department of Applied Chemistry and Biotechnology, Niihama National College of Technology
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YAMAUCHI Masahiro
Department of Polymer Chemistry, Kyoto University
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OKUMURA Yukihisa
Department of Polymer Chemistry, Kyoto University
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