Liposomal membranes. IX. Fluorescence depolarization studies on N-dansylhexadecylamine in liposomal bilayers.
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概要
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A fluorescent probe, <I>N</I>-dansylhexadecylamine (DSHA), was found to aggregate in aqueous media by monitoring the emission maximum, relative intensity, and polarization of fluorescence as functions of solvent polarity and probe concentration. In aqueous methanol, the <I>critical solvent polarity</I> for DSHA at a given concentration to form self-aggregates corresponded to 57% (v/v) aqueous methanol, while the <I>critical aggregate concentration</I> for the probe was 2.3×10<SUP>−6</SUP> M in 50% aqueous methanol. In liposomal bilayers, the dansyl moiety seemed to be localized in a polar region close to the surface of membranes. Fluorescence characteristics of DSHA in liposomes as a function of incubation temperature revealed the phase transition of liposomal bilayers, which was almost consistent with those previously estimated by other methods. As the salt concentration was increased up to about 1.5 M, DSHA in liposomes underwent the phase separation and formed self-aggregates even in liposomal bilayers.
- 公益社団法人 日本化学会の論文
著者
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SUNAMOTO JUNZO
Department of Applied Chemistry and Biotechnology, Niihama National College of Technology
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Sunamoto Junzo
Department of Industrial Chemistry, Faculty of Engineering, Nagasaki University
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Iwamoto Kiyoshi
Department of Industrial Chemistry, Faculty of Engineering, Nagasaki University
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