ラット肝isoferritin phenotypeに関する実験的研究-2-無細胞合成系によるラット肝ferritin messenger RNAの翻訳

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Poly (A+) rich messenger RNA were isolated from rat livers and translated in a cell-free synthesizing system derived from wheat germ S<SUB>30</SUB> and the following results were obtained.<BR>By this translation assay, the ferritin mRNA on polysomes from iron loaded rats gave approximately twice as much incorporation into ferritin as the mRNA from controls. In addition, the peptides corresponding to H- and L-subunits of carrier ferritin were obtained by immunoprecipitation using anti-rat liver ferritin antiserum and SDS/polyacrylamide gel electrophoresis, suggesting that these two subunits were primary gene products of ferritin synthesis.<BR>Comparing with the results of in vivo experiment (1st. of our paper), the preferential induction of L-subunits were not so remarkable and it was suggested that iron facilitates the translation efficiency of L-subunit messenger RNA and the assambly of L-subunits rather than H-subunits into ferritin molecule.
社団法人日本肝臓学会の論文