薬物アレルギ-性肝内胆汁うっ滞の発生機構に関する研究

概要

論文の詳細を見る
When peritoneal lymphocytes from patients with drug-induced allergic intrahepatic cholestasis were stimulated with a specific drug in vitro in the presence of soluble liver specific fraction and their culture supernatant was injected into the mesenteric vein of rats, a marked reduction of bile flow and bile acid excretion was seen in several cases. Similar results were obtained when lymph node lymphocytes from a sensitized guinea pig were stimulated with a specific antigen and its culture fluid was injected into the mesenteric vein of rats. Gel filtration of the active supernatant caused a reduction of bile flow which indicates that this active principle might have a molecular size similar to that of MIF. Histologically, a dilated bile canaliculus with diminution of the microvilli and vesicles around the dilated canaliculus were observed by electron microscopy after injection of culture supernatant into rats. No such changes were observed in rats after injection of the supernatant of a lymphocyte culture prepared from normal individuals in the same manner as described for patients or sensitized guinea pigs.<BR>These results strongly suggest that sensitized lymphocytes produce a factor (or factors) which cause intrahepatic cholestasis by stimulation with a specific drug-carrier antigen in patients with the intrahepatic cholestasis.
社団法人日本肝臓学会の論文