Characterization of 5-Hydroxytryptamine Receptors on the Isolated Pig Basilar Artery by Functional and Radioligand Binding Studies.
スポンサーリンク
概要
- 論文の詳細を見る
5-Hydroxytryptamine (5-HT)-receptor subtypes on pig basilar arteries were investigated by measuring the contractile responses to 5-HT agonists, the effects of antagonists on the responses and by carrying out a radioligand binding assay with [<SUP>3</SUP>H]5-HT. The rank order of contractile agonist potency (according to the pEC<SUB>50</SUB> values) was 5-carboxamidotryptamine ≤ 5-HT > α-methyl-5-HT > (±)-8-hydroxy-dipropylaminotetralin. The contractile responses were not affected by endothelial denudation, and the 5-HT-induced contractions were antagonized competitively by ketanserin. Methiothepin shifted the 5-HT concentration-response curves to the right and downwards in a concentration-dependent manner. In the presence of ketanserin (10<SUP>-6</SUP> M), however, methiothepin antagonized the 5-HT-induced contractions competitively. Specific [<SUP>3</SUP>H] 5-HT binding to 5-HT receptors was saturable, reversible and showed high (K<SUB>d</SUB>, 2.5 nM) and low (K<SUB>d</SUB>, 710 nM) affinities, with respective B<SUB>max</SUB> values of 29.5 and 1950 fmol/mg protein. These results indicate that both 5-HT<SUB>1</SUB> and 5-HT<SUB>2</SUB> receptors are present on pig basilar arterial smooth muscle cells, and their stimulation results in contraction.
- 公益社団法人 日本薬理学会の論文
著者
-
Miyamoto Atsushi
Department Of Cardiovascular Medicine School Of Medicine Hokkaido University
-
Nishio Akira
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
-
NISHIO Akira
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University
-
Sakota Toyoaki
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University
関連論文
- Participation of autophagy in the degeneration process of rat hepatocytes after transplantation following prolonged cold preservation
- A case of hepatocellular carcinoma with multiple lung, spleen, and remnant liver metastasis successfully treated by combination chemotherapy with the novel oral DPD-inhibiting chemotherapeutic drug S-1 and interferon-α
- EVIDENCE FOR CENTRAL ALPHA_2-ADRENERGIC MECHANISM OF CLONIDINE-INDUCED EJACULATORY DISTURBANCE IN DOGS
- Peak conditions of kyanite-bearing quartz eclogites in the Sanbagawa metamorphic belt, central Shikoku, Japan
- Rounded Atelectasis Associated with Pulmonary Lymphangioleiomyomatosis
- -295-CENTRALLY MEDIATED CARDIOVASCULAR EFFECTS OF DIGOXIN : Cardiotonics : PROCEEDINGS OF THE 42nd ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- -84- PROGNOSTIC IMPLICATION OF RIGHT AND LEFT VENTRICULAR DYSFUNCTIONS AND EXERCISE TESTING IN THE POST-MYOCARDIAL INFARCTION PATIENT : Congestive Heart Failure : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE C
- Vitamin K2 has growth inhibition effect against hepatocellular carcinoma cell lines but does not enhance anti-tumor effect of combination treatment of interferon-α and fluorouracil in vitro
- Association Between Recurrence of Hepatocellular Carcinoma and α-Fetoprotein Messenger RNA Levels in Peripheral Blood
- A Successful Resection and Long-Term Survival of a Patient with Intrahepatic Recurrences of Combined Hepatocellular-Cholangiocarcinoma : Report of a Case
- Liver Abscess Formation After Microwave Coagulation Therapy Applied for Hepatic Metastases from Surgically Excised Bile Duct Cancer : Report of a Case
- Interferon alpha receptors are important for antiproliferative effect of interferon-α against human hepatocellular carcinoma cells
- -264- A NEW THROMBOLYTIC THERAPY USING AUTOLOGOUS PLASMIN RICH PLASMA : Ischemic Heart Diseases : FREE COMMUNICATIONS(II) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- -P97- USEFULNESS OF RIGHT ATRIAL FUNCTION ANALYSIS IN THE PATIENTS WITH HEART DISEASE : RI : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- THE POTENTIATED THROMBOLYSIS UNDER SELECTIVE INFUSION OF AUTO PLASMIN RICH PLASMA (APRP) : PTCA, Thrombosis : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- 24)RIGHT VENTRICULAR DYSPLASIA : CLINICAL FEATURES COMPARED WITH DILATED CARDIOMYOPATHY : THE 8th CONFERENCE ON CARIDIOMYOPATHY
- THE POTENTIATED THROMBOLYSIS USING AUTOPLASMIN RICH PLASMA (APRP) UNDER SELECTIVELY BLOOD FLOW OBSTRUCTION TRANSIENTLY PRODUCED BY BALLOON-TIP CATHETER : Ischemic Heart Disease (III) : FREE COMMUNICATIONS (III) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC
- RIGHT AND LEFT VENTRICULAR FUNCTION DURING EXERCISE IN PATIENTS WITH CORONARY ARTERY DISEASE : Radio Isotope : 46th Annual Scientific Meeting, Japanese Circulation Society
- POTENTIATION OF TOXICITY AND POSITIVE INOTROPIC EFFECT OF OUABAIN BY FUROSEMIDE IN GUINEA PIG HEART
- Evaluation of the Left Ventricular Performance in Patients with Ischemic Heart Disease Using Radionuclide Angiocardiography : The Present Status of Cordiovascular Nuclear Medicine
- SENSITIVITY OF THALLIUM-201 IMAGING FOLLOWING PHARMACOLOGIC VASODILATION BY DIPYRIDAMOLE IN DETECTING CORONARY ARTERY STENOSIS : Isotope Imaging : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- The Non-invasive Evaluation of Regional Myocardial Ischemia Studied by a "Stress-injected" Thallium-201 Myocardial Imaging and a Radionuclide Left Ventriculography : Symposium on Myocardial Ischemia
- CHANGES OF HUMORAL AGENTS IN PHYSICAL EXERCISE BY BICYCLE ERGOMETER : IInd Auditorium : PROCEEDINGS OF THE 40TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- EFFECTS OF FUROSEMIDE AND SOLUDACTONE ON OUABAIN TOXICITY IN GUINEA PIGS : IInd Auditorium : PROCEEDINGS OF THE 40TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Liver transplantation for hepatitis C
- Endothelium-Dependent Relaxation in Response to Low Concentrations of Bradykinin Is Enhanced by Phosphoramidon, Bosentan and BQ-123 in Bovine Coronary Arteries In Vitro
- The Prevalence of Antimicrobial-Resistant Escherichia coli in Two Species of Invasive Alien Mammals in Japan
- Chromatographic Separation of Galactosylkojic Acid
- Successful outcome after resection of liver metastasis arising from an extraadrenal retroperitoneal paraganglioma that appeared 9 years after surgical excision of the primary lesion
- Multiple-Valued Constant-Power Adder and Its Application to Cryptographic Processor
- Mechanisms of the Enhanced Contractile Response to a Low Concentration of Phorbol 12, 13-Dibutyrate in Thoracic Aorta Isolated from Rats with Dietary Magnesium Deficiency
- THALLIUM-201 (Tl-201). TECHNETIUM-99m PYROPHOSPHATE (Tc-PYP) MYOCARDIAL IMAGING FOR ISCHEMIC HEART DISEASE : IVth Auditorium
- STUDIES ON CHRONIC TOXICITY OF THE LOW LEVELS OF O, O-DIMETHYL O-(3-METHYL-4-NITROPHENYL) PHOSPHOROTHIONATE (SUMITHION) IN THE RAT
- STUDIES ON THE DIFFERENCE IN THE BIOLOGICAL ACTIVITIES INDUCED BY PYROGEN IN RABBITS RESTRAINED BY TWO DIFFERENT METHODS
- CHANGES OF THE BODY TEMPERATURE OF RABBITS BY RESTRICTION IN PRONE OR SUPINE
- Multicenter Prospective Randomized Phase II Study of Antimicrobial Prophylaxis in Low-Risk Patients Undergoing Colon Surgery
- Structure of the Novel Insecticidal Sesquilignan, Haedoxan A(Organic Chemistry)
- INCREASE IN POTASSIUM RELEASE FROM RAT PAROTID TISSUE REPEATEDLY EXPOSED TO ISOPROTERENOL
- Cyclosporin A in the Treatment of Acute Exacerbation of Idiopathic Pulmonary Fibrosis
- Efficacy of Gefitinib for Elderly Patients with Advanced Non-Small Cell Lung Cancer Harboring Epidermal Growth Factor Receptor Gene Mutations: A Retrospective Analysis
- Modulation of Rabbit Platelet Aggregation and Calcium Mobilization by Platelet Cholesterol Content
- Conditions for "Coronary Steal" Caused by Coronary Vasodilator in Man
- Interstitial pneumonia induced by combined intraarterial 5-fluorouracil and subcutaneous interferon-α therapy for advanced hepatocellular carcinoma
- Pulmonary Tumor Thrombotic Microangiopathy: A Clinical Analysis of 30 Autopsy Cases
- Hereditary Hemorrhagic Telangiectasia with Pulmonary Arteriovenous Malformations and Embolic Strokes Treated Successfully with Video-assisted Thoracoscopic Resection
- A Case of Concurrent Sarcoidosis, Aortitis Syndrome and Crohn's Disease
- Vasomotor effect of histamine on pig and cattle coronary artery in vitro.
- Increase of .ALPHA.1-adrenoceptor binding after exposure to isoproterenol in rat parotid glands.
- .BETA.1-Adrenergic regulation of cyclic nucleotide levels and potassium fluxes in rat parotid gland.
- Isoproterenol inhibition of potassium release from rat parotid gland.
- Potential thrombolysis under selective infusion of autologous plasmin(AP) solution.
- Alterations in myocardial perfusion during painless ST-segment depression in patients with angina pectoris.
- Myocardial perfusion and left ventricular performance during exercise-induced ST-segment depression in apparently healthy subjects.
- Histamine content and histidine decarboxylase activity in the spleen of the magnesium-deficient rat: Comparison with the skin and peritoneal mast cells.
- Characterization of .BETA.-Adrenoceptors in Pig Basilar Artery from Functional and Radioligand Binding Studies.
- Characterization of 5-Hydroxytryptamine Receptors on the Isolated Pig Basilar Artery by Functional and Radioligand Binding Studies.
- Diuretics aggravate zinc deficiency in patients with liver cirrhosis by increasing zinc excretion in urine