CENTRAL ACTION OF BRADYKININ (II) SEPARATION OF BRADYKININ DEGRADING ENZYME FROM THE RAT BRAIN

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概要

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• Separation of kininases from the rat brain and identification of the products produced by these enzymes were examined using DEAE-cellulose column chromatography and thin-layer chromatography, respectively. Fraction A (F-A), which was developed with 70 mM NaCl, released Arg-Pro-Pro-Gly-Phe and Ser-Pro-Phe-Arg, fraction B (F-B), with 100 mM NaCl, cleaved the Pro<SUP>7</SUP>-Phe<SUP>8</SUP> and Phe<SUP>8</SUP>-Arg<SUP>9</SUP> peptide bonds of the bradykinin molecule and fraction C (F-C) with 150 mM NaCl hydrolyzed bradykinin to amino acids. The specific activity of each fraction determined by the bioassay system was 158.7, 51.0 and 14.8 nmole bradykinin/mg protein/ min, respectively. The intensities of their fluorescence on the chromatogram showed visibly that o-phenanthroline effectively inhibited F-A, B and C at a concentration of 0.5 mM. These results suggest that heterogeneous enzyme systems are present in the rat brain and regulate the levels of bradykinin in the brain.

• 社団法人 日本薬理学会の論文

著者

• Yamauchi Aiko

  Department Of Pharmaceutical Information Science Graduate School Of Pharmaceutical Sciences Universi

• Kariya Kimio

  Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University

• TSUDA Yuko

  Department of Analytical Toxicology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University

• Okada Yoshio

  Department Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences Kobe-gakuin University

• IWAKI Hideo

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University

• OKAMOTO Midori

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• IWAKI Hideo

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• YAMAUCHI Aiko

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• KARIYA Kimio

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• TSUDA Yuko

  Department of Chemistry, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• OKADA Yoshio

  Department of Chemistry, Faculty of Pharmaceutical Sciences, Kohe-Gakuin University

• OKADA Yoshio

  Department of Chemistry, Faculty of Engineering Science, Osaka University

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