DETERMINATION OF KININ IN THE RAT BRAIN BY A SENSITIVE RADIOIMMUNOASSAY
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概要
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Kinin level in the rat brain was determined using a highly sensitive radioimmunoassay with a purified tracer. Monoiodinated Tyr^8-bradykinin (BK) was separated from the non-iodinated peptide by paper chromatography, using 0.2M ammonium acetate as the developer. This purification method resulted in a ligand with a highly specific radioactivity (2200 μCi/nmol), which raised the radioimmunoassay sensitivity about 5-fold and enabled to determine the brain kinin level in the rat. The brain extract competed with iodinated antigen for an antibody binding site in the same manner as did authentic BK, indicating the immunological idintity of them. Brain kinin level was estimated to be 139±79 fmol BK eq/g (n=7) in an adult male rat of the Sprague-Dawley strain.
- 公益社団法人日本薬学会の論文
著者
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Yamauchi Aiko
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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Yamauchi Aiko
Department Of Pharmaceutical Information Science Graduate School Of Pharmaceutical Sciences Universi
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Kariya Kimio
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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NAKAYAMA ATSUMU
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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Nakayama Atsumu
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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