Gel Filtration Makes a Nonincinerative Assay of Thyroxine Available for Chicken Plasma

概要

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The idea of using nonincinerative procedures for plasma thyroxine has been introduced in the field of clinical chemistry. The analytical procedures for iodine determination in organic compounds exploit the iodide-catalyzed redox reaction which is called a thiocyano-nitrite reaction. The methods involve the use of ion-exchange column chromatography to effect the separation of thyroid hormones from other plasma iodine constituents.Thyroid hormones are quantitated in acetic acid column eluates by the destruction of organic matter and the release of inorganic iodide, which is determined photometrically by the thiocyano-nitrite reaction. Since triiodothyronine (T3) cannot be separated from thyroxine (T4) by the ion-exchange column chromatography, the determined value shows the total amount of thyroid hormones.However, as the T3 concentration in the plasma of most animals is much lower than that of T4, the value can be regarded as T4 in a practical sense. Recently, a clinical T4 assaying kit, which is based on the idea mentioned above, has been commercialized and widely used in Japan.However, T4 in chicken plasma cannot be measured by this kit because unknown substance (s) which interferes the redox reaction is eluted together with T4 from the ion-exchange resin column.Therefore, we tried to eliminate the interfering substance (s) from the plasma by gel filtration to make the kit applicable to chicken plasma.
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